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Chaudhary, H. K.
- Effects of Different Growth Media on Germinability of Interspecific Hybrids between Vigna mungo< and V. umbellata
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Authors
Affiliations
1 Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
1 Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
Source
Himachal Journal of Agricultural Research, Vol 46, No 1 (2020), Pagination: 29-35Abstract
A total of 15 interspecific crosses involving five genotypes of urdbean (Vigna mungo) and three genotypes of ricebean (V. umbellata) were attempted to study the effects of different growth media on hybrid germination. Since the seeds obtained from the interspecific crosses did not germinate under normal soil conditions so attempts were made to grow them on various media i.e. Salt solution, Half and full strength MS medium, MS basal medium and Gamborg B5 media. Seeds were able to germinate only on the salt solution. F1 seeds obtained from cross Him Mash-1 x VRB-3 showed maximum response on salt solution with respect to radicle formation (44.60 per cent) followed by Palampur-93 x PRR-2 (38.30 per cent) & Him Mash-1 x PRR-1 (23.89 per cent).Cross Palampur-93 x PRR-2 (17.02 per cent) produced maximum number of plantlets followed by Him Mash-1 x VRB-3 (15.32 per cent). The study indicated that different kinds of post fertilization barriers such as retarded embryo & endosperm development, production of inviable & shriveled seed, inability of seed to form radicle and yellowing of plantlets are responsible for complete lethality. The crosses showing substantially high per cent of germination can be utilized for genetic improvement of urdbean.Keywords
Interspecific Hybridization, Germinability, V. mungo, V. umbellata.Full Text
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- Screening of Potential Wheat Genotypes for Drought Tolerance using Polyethylene Glycol
Abstract Views :406 |
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Authors
Affiliations
1 Molecular Cytogenetics and Tissue Culture Lab (MCTL), Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
2 Vice-Chancellor, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
1 Molecular Cytogenetics and Tissue Culture Lab (MCTL), Department of Genetics and Plant Breeding, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
2 Vice-Chancellor, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176 062, IN
Source
Himachal Journal of Agricultural Research, Vol 46, No 2 (2020), Pagination: 130-135Abstract
Screening for early drought tolerance by using three treatments of PEG-6000, control (T1), 15% (T2 ) and 25% (T3) was employed in six wheat genotypes (DH 114, DH 40, DH 65, C 306, DH 52 and DH 5). The genotypes were 3 evaluated for shoot length, coleoptile length, ischolar_main length and ischolar_main to shoot ratio. From the results, shoot, coleoptile and ischolar_main length decreased with increased PEG concentrations. DH 40 expressed highest shoot length in T and T with least reduction and C 306 witnessed longer coleoptile with least reduction over control. DH 114 2 3 expressed highest ischolar_main length in all treatments, while DH 40 had least reduction (3.31 and 7.96% in T2 and T3, respectively). C 306 in T3 and DH 114 in T2 were superior for ischolar_main to shoot ratio. DH 40, DH 114 and C 306 3 2 possessed early drought tolerance behaviour and might be productive in wheat improvement programmes.Keywords
Coleoptile Length, DH 40, Drought Tolerance, PEG.Full Text
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